| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Traub, Celia B (8)
|
MAPAAC |
36.20 |
37.60 |
1.40 |
| 2 |
Doran, Erika L (8)
|
MAUN |
40.63 |
38.77 |
-1.86 |
| 3 |
Kellner, Gabriella H (7)
|
MACBST |
|
39.42 |
- |
| 4 |
Davis, Elisabeth A (8)
|
MATSS |
|
43.33 |
- |
| 5 |
Adamski, Hannah B (8)
|
MATSS |
|
43.75 |
- |
| 6 |
Jones, Gabrielle V (8)
|
MAUPST |
|
44.46 |
- |
| 7 |
Pappentick, Addison K (6)
|
MAEMAC |
46.55 |
45.73 |
-0.82 |
| 8 |
Robinson, Grace C (7)
|
MAEMAC |
46.18 |
46.16 |
-0.02 |
| 9 |
Chukoskie, Emily P (8)
|
MAPAAC |
46.12 |
46.90 |
0.78 |
| 10 |
Price, Katryna M (7)
|
MAEMAC |
47.93 |
47.56 |
-0.37 |
| 11 |
Shutkufski, Madison H (7)
|
MAEMAC |
48.54 |
49.28 |
0.74 |
| 12 |
Tyson, Teagan P (7)
|
MATSS |
|
50.45 |
- |
| 13 |
Farag, Hanna (8)
|
MAPJA |
58.54 |
56.01 |
-2.53 |
| 14 |
Sroka, Natalie (6)
|
MAPJA |
|
58.32 |
- |
|
Johnson, Isabelle C (8)
|
MAEMAC |
|
NS |
- |